Integral Control System

All control software is within the instrument, tamper proof, providing easy menu driven operation with massive safe storage for scans, plots, curves etc. PC control and data transfer to a PC are provided.

Equal Sample and Reference Facilities

Identical and generous sample and reference sampling facilities widens the scope of measurement, for example, to enable multi-cell sampling in both beams and accommodate bulky accessories in the reference beam.

Method Operation and Storage

Up to 30 sets of instrument parameters, including concentration curves may be stored, security code protected. When recalled, instrument parameters are automatically set, avoiding errors.

Alpha Numeric Entry

Full Alpha Numeric entry is provided so that results may be fully documented for GLP. Formula entry enables the creation of a wide range of formulae for automatic calculations using many wavelengths.

Software Enhancement By E-SEF

E-SEF - Encoded Software Enhancement Facility - allows software modules to be enabled using encoded numbers provided by telephone, fax or E-mail. Software requirements may be tailored, at any time, as required.

Data Storage and Protection

Data, including 100 spectra, concentration curves , kinetics and time course plots may be stored in safe memory, security code protected against tamper or deletion using a four digit password.

Reprocessing

Scans, time course plots, and reaction curves may all be reprocessed to any required format on the display screen and then plotted on the integral or an external printer.

Performance Validation

Software and certified standards enable wavelength accuracy, Absorbance accuracy, bandwidth etc. to be validated. A timed and dated report includes the serial number of the instrument.
Quantification

Powerful Quantification

Many quantitative methods are available including band area computation with background correction, differencing and stripping of spectra, wine and colour assays, mathematical calculations between several wavelengths, protein and DNA assays.

Curve and Line Fitting

A linear, quadratic or cubic concentration curve may be fitted to up to 30 standards, with or without an intercept at the origin. Standards may be deleted and replaced and a new curve fitted.

Reaction Kinetics

Single cell kinetic measurements are possible with plots and data displayed on screen. Data may be reprocessed, regression analysis applied and curves stored, security code protected against tamper or deletion.

Multi-Cell Reaction Kinetics

Up to four or six cells are automatically measured with all reaction curves displayed on screen. Each curve may be selected for processing with the results of regression analysis printed.

Multi Wavelength Assays

Assays are provided using either two or three wavelengths. A wine colour assay is provided which may use up to ten different wavelengths. Computations at up to four wavelengths are also provided.

DNA and Protein Assays

Protein assays may be made using either the 2 or 3 wavelength methods of Warburg and Christian. Also programmed are techniques and analytical procedures for assays by Lowry, Bradford, Biuret and BCA methods.

Band Area and Height Computation

Band areas with correction for sloping background absorption are readily quantified, with all details and construction displayed. Band areas may also be computed for derivative spectra to facilitate difficult quantification.

Wavelength Program

Using the various cell changers, measurements may be made on up to 4, 6 or 8 cells at a single wavelength with cycle time and number of cycles entered by the operator. A cell and wavelength program may be combined.

Cell Program

Measurements may be programmed for 10 different operator selected wavelengths with the cycle time and number of cycles selected by the operator. A wavelength and cell program may be combined.
Scanning

Double Beam Wavelength Scans

cans at up to 4000nm/min are presented on screen or printed with all peaks annotated with wavelength and absorbance. Tables are also presented listing all peaks and troughs with values.

Display Scrolling

Use of the display scrolling facility enhances the effective screen size and enables spectra and data up to 550nm wide to be viewed.

Dynamic Scan Recall

The last eight scans are automatically retained for recall by entering their scan sequence number and may be reprocessed, overlayed or transferred to protected memory for future recall.

Overlayed Spectral Scans

Up to eight scans, including stored scans, may be overlayed, with or without offsets for convenient comparisons or further manipulation.

Difference Spectra

New wavelength scans or scans from store may be presented on screen and the difference spectra produced. The scans may be normalised enabling spectral stripping to reveal impurities or additives.

Derivative Spectra

Derivative spectra, up to fourth are readily produced, with selectable smoothing, either alone or superimposed on the fundamental, with or without an offset. Scaling is automatic with all peaks and troughs quantified.

Scan Storage

Up to 100 spectra may be stored in 10 safe memory banks for future recall. Each spectrum is automatically allocated a recall number, when stored, and may be security code protected against tamper or deletion.

Programmed Scans

Automatic scans, single or repeat may be made of a cell program of up to 4, 6 or 8 cells. A programmed sequence of scans may also be made of a single sample.

Time Course Plots

Changing samples or flows may be examined and displayed by making a time course plot. Data may be reprocessed and stored, security code protected, with an automatically assigned recall number.
Sampling

Sampling Flexibility

The design of the double beam optical sampling allows exactly similar space and facilities in both the sample and reference beams.  Full size accessories and multi-cell reference sampling, with temperature control is therefore possible.  Operation is not limited to a single standard cell in the reference beam as in some other instruments.

Reflectance/Gel Scanning

Accessories include fixed angle and variable angle reflectance units and a gel scanning system for gels up to 100mm long.

Multi-User Convenience

Aquarius double beam instruments were designed for the greatest convenience in a laboratory where multi user operation is required.  All methods, stored scans, plots, curves and data remain in secure integral memory, tamper protected by passwords, readily accessible to all operators without the possibility of mislaid disks or plug in card modules.

Cells and Flowcells

As well as 10mm cells and flowcells, holders are available for rectangular and cylindrical cells up to 100mm pathlength. A microsampling system measures to 50ml or below.

Automatic Cell Changers

Automatic changers are available for 4, 6 or 8 cells, providing rapid sample changing or the facility for full programming.

Sipette Sampling

Samples down to 300 microlitres may be measured in a 10mm cell. Sampling is under control of the instrument's microprocessor system. Samples may be saved or pumped to waste.

Ultra-Micro Sampling

Valuable samples, such as biological samples prepared with costly reagents, may be measured using a special ultra-microcell and holder. With the system, samples of 50µl or even less may be accurately measured.  Third party nano cells may also be used.

Sample Temperature Control

Water and thermoelectric temperature control are available for single cells and automatic cell changers. Temperature is set from the control panel and displayed on the screen.

Dissolution Testing

Versions of the instruments fitted with a special cell compartment with piping port, are available for dissolution testing using Cecil TD software and a PC. Six and eight cell changers are available.

Batch Sampling

Automatic batch sampling for up to 100 samples is available. The pump fits within the sample compartment and volume etc is set from the control panel and displayed on screen. Batch number entry is provided.

Specular Reflectance

Special accessories enable specular reflectance measurements to be made. Both fixed angle and variable angle reflectance accessories are available. The latter is particularly useful for film thickness measurements.

Gel Scanning

A gel scanner enables gels up to 100mm long to be measured. An autoradiograph film holder is also available.

Micro - Sipette Sampling

Small samples or multiple samples are conveniently measured using the micro-sipette system, with very low cross contamination.  All measurements are made in the same cell, enhancing accuracy.  The pump, which is mounted in the sample compartment, operates under the control of the instrument's micro-processor system.
Assay Programmes

Reaction Kinetics

Both single cell and multi cell Kinetics are available with reaction plots to screen and printer. Regression analysis is applied to the user selected portion of reaction curves and results are reported in Units/Litre. Data may be reprocessed as required and curves stored. Sample temperature control is available for single cells and four and six cell changers.

Advanced Kinetics

An example of advanced kinetics is shown below where time based reaction data generated by the spectrophotometer, has been transferred into Grafit, fitted to a curve and transformed into a more suitable format for mathematical interpolation and calculation. In this particular example, the basic data plot can yield the limiting rate (Vmax), the Michaelis constant (Km) and the initial linear portion (Vmax/Km).

This has been transformed into the Eadie-Hofstee format and plotted to give better precision in the calculation of these important factors and constants.

Other transformations are available such as Lineweaver-Burk and the Hill plot for allosteric enzyme systems.

PC Data Manipulation

Using DataStream software, data may be transferred to a PC for use with spread sheets and other mathematical software packages.

More advanced reaction kinetics data manipulation may be readily performed with Grafit software. Linear, non-linear or polynomial curve fitting is easily accomplished together with non-linear regression analysis. A wide selection of standard equations are provided, together with an equation editor for customised equation fitting.

Data may also be transformed into other mathematical formats for plotting and calculation.

Band Quantification

Accurate quantitative assays using band area or height, corrected for background absorption, are exceptionally easy to carry out with the Series 7000 Aquarius instruments.

Analytical results are displayed on the screen with construction lines enabling the quality of the parameters to be assessed.

The procedure may be stored as a method with security code protection in the integral memory of the instrument.
   
Validation

Assessment of instrument performance is readily carried out using the validation software module built into all Aquarius instruments and released by E-SEF, 'encoded software enhancement facility'. Laboratories subject to regulatory control, or following G.L.P. are provided with a range of Pharmacopoeia (EP) tests as well as tests using filters traceable to national standards with certificates of calibration issued by Cecil Instruments. A selection of the tests are shown here.

Absorbance Accuracy

The Pharmacopoeia (EP) test for Absorbance accuracy, using a 60mg/L solution of Potassium Dichromate in a 10mm pathlength cuvette, is shown here.

Optical Bandwidth

The optical bandwidth of the instrument may be validated by measuring the half height width of the narrow deuterium emission line at 656.1nm. The measurement is carried out automatically.

Validation Using Filters

Absorbance accuracy is most conveniently measured using certified filter sets calibrated by Cecil Instruments against NPL certified standards. A validation printout for the procedure is shown here. Wavelength calibration is also carried out using certified filters.
======================================

系统功能







双光束光学系统

完全对称的光学系统可以同时测量样品和参比的最终准确度，精密度和基线平直度。长期稳定性达到比单波束系统好十倍。




积分控制系统

所有的控制软件就是仪器内，防篡改，提供简单的菜单驱动操作进行大规模的安全存储扫描，绘图，曲线等PC控制和数据传输到PC提供。




等于样品和参考设备

相同大方样品和参比采样设施加宽的测量范围，例如，为了使多细胞采样在两梁和容纳笨重配件参考光束中。




方法操作和储存

多达30套的仪器参数，包括浓度曲线可以存储，安全代码的保护。当回忆说，仪器参数都自动设置，避免错误。




字母数字输入

提供完整的字母数字项，这样的结果可能会完全记录的GLP 。公式输入支持创建各种公式使用多个波长自动计算。




软件增强通过E- SEF

E- SEF - 编码软件增强基金 - 允许使用通过电话，传真或电子邮件提供的编码数字被激活的软件模块。软件要求可定制的，在任何时候，根据需要。




数据存储和保护

数据，其中包括100光谱，浓度曲线，动力学和时间进程曲线可以存储在安全的存储，使用一个四位数的密码安全密码保护，防止篡改或删除。




后处理

扫描，时间过程图，并反应曲线可以全部进行再加工，以在显示屏幕上的任何所需的格式，然后绘制在积分或外部打印机。




性能验证

软件和认证标准，使波长准确度，吸光度精度，带宽等进行验证。一个定时及于报告包括仪器的序列号。
定量




强大的定量

许多定量方法可供选择，包括带面积计算与背景校正，差分和光谱，酒和彩色分析，几个波长，蛋白质和DNA检测的数学计算的剥离。




曲线和拟合线

线性，二次或三次浓度曲线可装配到高达30标准，使用或不使用截距在原点。标准可能会被删除并替换和新的曲线拟合。




反应动力学

单细胞动力学测量是可能的情节和画面上显示的数据。数据可能会被重新处理，回归分析应用和曲线存储，安全代码保护，防止篡改或删除。




多细胞反应动力学

多达四个或六个细胞与屏幕上显示的所有反应曲线自动测量。每条曲线可用于处理被选择与回归分析印刷的结果。




多波长测定

使用两个或三个波长的光被提供的检测。此酒颜色鉴定提供一种可使用多达十个不同的波长。还提供计算在多达四个波长。




DNA和蛋白质测定

蛋白质检测可以使用华宝和基督教无论是2或3波长的方法进行。也编有技术和实验分析程序被凯尔 - 洛瑞，布拉德福德，缩二脲和BCA方法。




带面积和高度计算

与校正倾斜的背景吸收带区域容易量化，并显示所有的细节和建设。带区域也可以被计算为微分光谱，以便难以定量。




波长程序

使用各种细胞变换器，测量可以由多达4或6或8个细胞在含有周期时间和由操作者输入的周期数的单一波长。的细胞和波长程序可被组合。




细胞程序

测量可以被编程为10个不同的操作员所选择的波长的周期时间和由操作者选定的周期数。波长和细胞程序可被组合。
扫描




双光束波长扫描

罐高达4000nm/min呈现在屏幕上或印有注释的波长和吸收所有的山峰。表中还提出，列出所有的波峰和波谷与价值观。




滚动显示

使用显示滚动设施的提高有效屏幕尺寸，使光谱和数据高达550nm的宽来看待。




动态扫描召回

八强扫描通过输入其扫描顺序号自动保留和召回可能被再加工，重叠或转移到受保护的内存以备将来调用。




重叠光谱扫描

多达八个扫描，包括存储的扫描，也可以重叠，带或不带偏移量为便于比较，或进一步的操作。




差谱

从存储新的波长扫描或扫描可以被呈现在屏幕上，所产生的差示光谱。该扫描可以被归一化使得频谱剥露出的杂质或添加剂。




导数光谱

微分光谱，上升到第四易于生产的，具有可选的平滑处理，无论是单独或叠加在基本的，带或不带偏移量。缩放是自动与量化所有的波峰和波谷。




扫描存储

高达100光谱可以存储在10安全存储银行以备将来调用。每个光谱是自动分配的召回数，存储时，也可以安全码保护，以防止篡改或删除。




编程扫描

自动扫描，单次或重复可以由一个单元程序的最多4个， 6个或8个细胞。扫描的编排顺序也可以由一个单一的样本。




时间课程地块

更换样品或流量可检测并显示通过做一个时间过程的情节。数据可能会重新处理和存储，安全代码保护自动分配的召回数量。
采样




采样灵活性

双光束光采样的设计允许在这两个样品光束和参考光束完全相似的空间和设备。因此，全尺寸的配件和多单元格引用的采样，随温度的控制是可能的。操作不限于参考光束在单细胞水平在某些其他工具。




反射/凝胶扫描

附件包括固定角度和变角反射单元和凝胶可达100mm长的凝胶扫描系统。




多用户便利

水瓶座双光束仪器被设计为在需要的多用户操作实验室最大的便利。所有的方法，存储扫描，图形，曲线和数据留在安全的整体记忆，篡改受密码保护，容易接触到所有运营商没有放错盘的可能性或插入卡模块。



 
细胞和测量管

以及10毫米细胞和测量管，支架可用于矩形和圆柱形电池可达100mm光程。一个微量进系统的措施至50ml以下。




自动换电池

自动换可用于4,6或8个细胞，提供快速样品更换或设施进行全面编程。




Sipette采样

样品下降到300微升可以以10mm比色皿进行测定。抽样是根据仪器的微处理器系统的控制权。样品可以保存或抽浪费。




超微型采样

有价值的样品，如制备昂贵试剂的生物样品，可以使用特殊的超微小区和保持器测定。用系统中， 50μl的或什至更少的样品可以被精确地测量。第三方纳米细胞也可使用。




样品温度控制

水和热电温度控制可用于单个细胞和细胞自动换。温度从控制面板中设置和显示在屏幕上。




溶出度测试

配有特殊的细胞室与配管口的工具版本，可使用塞西尔TD软件和PC溶出度测试。六到八个细胞兑换服务。




批量采样

自动批量采样多达100个样品是可用的。样品室中的泵适合，体积等从控制面板中设置并在屏幕上显示出来。提供批号条目。




镜面反射

特殊配件使镜面反射率测量作出。两个固定角度和变角反射附件可供选择。后者是用于薄膜厚度测量时特别有用。




凝胶扫描

凝胶扫描仪使凝胶可达100mm长的时间来衡量。放射自显影胶片支架也可以。




微 - Sipette采样

小样品或多个样品用微sipette系统可方便地测得，具有非常低的交叉污染。所有的测量都在相同的细胞制成，提高精度。泵，其被安装在样品室中，操作该仪器的微型处理器系统的控制下。
试验计划




反应动力学

单细胞和多细胞动力学可与反应阴谋屏幕和打印机。回归分析应用到反应曲线和结果的用户选择的部分中报告单位/升。根据需要和曲线存储的数据可以被重新处理。样品温度控制可用于单细胞和四和六单元换。




高级动力学

先进的动力学的例子所示，其中由分光光度计产生基于时间的反应数据，已转移到GRAFIT ，装到一个曲线并转化为一个更合适的格式为数字内插和计算。在这个特定的例子中，基本的数据图可以得到限流速率（Vmax ） ，米氏常数（公里），并在初始线性部分（速度/公里） 。




这已转化为伊迪 - Hofstee格式和密谋提供更好的精度在这些重要因素和常数的计算。




其他转换可如双倒数和积山的别构酶系统。




电脑数据操作

使用的DataStream软件，数据可传输到电脑与电子表格和其他数学软件包的使用。




更高级的反应动力学数据操作可以很容易地进行与GRAFIT软件。线性，非线性或多项式曲线拟合很容易用非线性回归分析来实现在一起。提供了多种选择的标准方程，连同一个公式编辑器定制方程拟合。




数据也可以转换成其他的数学格式绘制和计算。







带定量

使用带面积或高度准确的定量分析，为背景吸收校正，是非常容易执行与7000系列水瓶座仪器。




分析结果显示在屏幕上，建筑线条使被评估参数的质量。




该程序可以被存储为与安全码保护仪器的积分存储器的方法。
    
验证




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仪器性能评估是很容易进行了使用内置到所有水瓶座仪器和电子海基会， “编码软件增强工具”发布了验证软件模块。实验室受监管控制，或以下的GLP提供了一系列药典（ EP）的测试，以及测试使用过滤器可溯源至国家标准与塞西尔仪器发出的校准证书。一个选择的测试都在这里显示。




吸光度准确度

药典（ EP）测试吸光度准确度，用重铬酸钾的60毫克/ L的溶液采用10mm光程的比色皿，在这里显示。







光学带宽

仪器的光学带宽可以通过测量窄氘发射线的半高宽在656.1nm进行验证。测量自动进行。




验证使用过滤器

吸光度准确度使用由塞西尔仪器对不良贷款认证标准校准认证的过滤器设置最方便的测量。一个验证打印输出的程序如下所示。波长校准也进行了使用认证的过滤器。

 技术规格：

Optical System	Fully symmetrical double beam optical system. All mirrors coated for long life
Monochromator	Modified Czerny-Turner design using 1200 L/mm holographic diffraction grating
Detectors	Two silicon diode detectors
Self Test and Calibration	Automatic at switch on
Wavelength Range and Accuracy	190 - 1100nm; 0.5nm  (For CE 7200, 190 - 900nm; 0.5nm)
Wavelength Reproducibility	0.1nm
Wavelength Readout	Digital display on screen
Wavelength Selection	By GOTO Key and keypad entry
Optical Bandwidth	For CE 7200 and CE 7400 1.8 nm.    For CE 7500 user selectable, 0.5, 1, 1.8 and 4 nm.  For CE 7400S 1 nm
Stray Light	Less than 0.02% at 220nm, NaI; and 0.02% at 340nm, NaNO2
Display Screen	Large backlit LCD displays spectra, menus, data, operator prompts and instrument status.
Derivative Spectra	1st to 4th derivatives - autoranged or operator defined.
Autozero	Automatic by press button
Photometric Readout	Digital display of A, %T and C on screen
Photometric Accuracy	±.004A at 1A
Photometric Noise	Less than ±0.0001A at 550nm
Baseline	Stored in safe memory
Baseline Flatness	±0.001A (300nm/min, 200-900nm)
Baseline Stability	Better than ±0.0001A/hour
Computer Interface	Bi-directional RS 232  and USB ports
Scan Speeds	1-4000nm/min
Wavelength Scale Expansions	1-100nm/cm
Method Storage	30 methods may be stored and retrieved
Method Protection	Each protected by 4 digit password
Reaction Kinetics	With plots, reprocessing of data as required, for single cell or up to 6 cells
Overlaid Scans	Derivatives and scans superimposed with or without offset. Up to 8 scans may be overlaid.
Cell Programming	Up to 4, 6 or 8 cells
Programme Cycles	From 1 to 9999
Programme Delay (cycle time)	From 0 seconds to 4 days (1 sec resolution)
Wavelength Programming	Up to 10 wavelengths. May be combined with a cell programme
Storage of Spectra	100 spectra may be stored security code protected
Concentration Curve Fitting	Linear, quadratic or cubic with editing of standards. Up to 30 curves may be stored and protected
Batch Sampling	For up to 100 samples under internal control with screen display
Date and Time Information	Printed from real time clock
Power Requirements	110-250V, 50/60Hz, 200 watts.
Dimensions	635 x 410 x 210mm
Weight	33kg

CE 7200 Spectrophotometer

1.8 nm Optical bandpass. Wavelength range 190-900nm.

CE 7260 Dissolution Spectrophotometer

Suitable for tablet dissolution monitoring using a choice of dissolution software and an automatic six or eight cell changer.

CE 7400 Spectrophotometer

1.8 nm Optical bandpass. Wavelength range 190-1,100nm.

CE 7460 Dissolution Spectrophotometer

Suitable for tablet dissolution monitoring using a choice of dissolution software and an automatic six or eight cell changer.

CE 7400S Spectrophotometer

1 nm Optical bandpass. Wavelength range 190-1,100nm.

CE 7460S Dissolution Spectrophotometer

Suitable for tablet dissolution monitoring using a choice of dissolution software and an automatic six or eight cell changer.

CE 7500 Spectrophotometer

User selectable 0.5, 1, 1.8 and 4 nm Optical bandpasses. Wavelength range 190-1,100nm.

CE 7560 Dissolution Spectrophotometer

Suitable for tablet dissolution monitoring using a choice of dissolution software and an automatic six or eight cell changer.